Re-Introduction of Soil Mycorrhizae into Roadside Prairie Planting

The three objectives of this project were as follows: (1.) to survey the arbuscular mycorrhizal fungi from the prairies, wetlands, and Mn/DOT experimental sites in anticipation of using data as indicators of the sites' health, (2.) to produce mycorrhizal inocula by different methods and (3.) to incorporate the inoculum into a Mn/DOT restoration site. This project was the first year of a three year study that seeks to define the variability in the
mycorrhizal spores and other structures present in different ecosystems and determine if these fungi can be used as indicator species.

All phases of the project were successful. The preliminary results suggest the mycorrhizal fungal spore numbers will be useful health indicators of restoration sites. General and single spore mycorrhizal inocula were generated using primarily native grasses and forbs as plant hosts. The general inoculum was then incorporated into a Mn/DOT experimental site. Future evaluations of this site will help to answer long-term questions regarding the possible benefits of amending disturbed roadway plantings or other prairie restorations with mycorrhizal inoculum.