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This report describes a long-term study to monitor and evaluate the ecosystem recovery of seven wetland restorations in south central Minnesota. The study looks at the impact of planting on wetland restoration success in inland wetlands and develops a methodology to assess wetland restoration success. The study focused on the following question: To what extent can revegetation stimulate overall biological recovery in isolated restoration sites? Researchers used aspects of ecosystem function and structure to characterize recovery rates in planted and unplanted restored sites relative to one another and to reference wetlands, as well as indices of biotic integrity for plants, invertebrates, birds, and amphibians in addition to other indicators of ecosystem structure, such as soil organic matter and water chemistry. Researchers developed field protocols, created biotic indices, and established baseline monitoring on each site including an as-built topographic survey, installation of groundwater and surface water stations, characterization of soils, vegetation, and vertebrates. A long-term monitoring system is now in place that will allow for the assessment of ecosystem recovery of seven restored wetlands relative to four reference wetlands.

This report presents the results of a four-year study on techniques for revegetation of native sedges in created basins. Although often the dominant genus in shallow wetlands, sedges (Carex spp.) do not readily recolonize after restoration or creation of the water table. It is unlikely that sedges will naturally establish in created wetlands. The results of seed germination studies on five Carex species suggest the highest germination rates in fresh seeds - with one exception. Wet/cold storage also can prolong seed viability for at least two-and-a-half years. Dry storage is not recommended for wetland sedge seeds. Short-term wet/cold treatment after prolonged dry storage does not improve germination rates. Sensitive to deep water, rising water levels, and competition during the establishment year, seedlings grew well across a wide range of water depths in subsequent growing seasons. Both species outcompeted annual weeds within two to three growing seasons, but not Phalaris arundinacea (reed canary grass). The study recommends weed control during the establishment year to prevent the invasion of P. arundinacea. Wetland soil promotes seedling growth relative to other soils, but does not affect germination rates. Because of the potential for the introduction of undesirable weeds, the study does not recommend the use of donor wetland soil. Instead, study results suggest the potential for the use of organic top-dressings.

Reduction in phosphorus is critical because phosphate, a dissolved form of phosphorus, sustains algal and cyanobacteria growth and causes a wide range of water-quality impairments in the ponds and downstream waters including algal blooms, excess floating plants, taste, and odor problems. Many stormwater ponds and wetlands that treat stormwater appear to be less effective than expected or originally intended in phosphorus retention, a key function of these ponds in urban environments. There is evidence that many old ponds are releasing phosphorus from bottom sediments at high rates and likely exporting phosphorus to downstream surface water bodies. A major outcome of this project is a pond Assessment Tool to assess the risk of high phosphorus concentrations in ponds and sediment release of phosphorus. The tool is based on 20 ponds with detailed water quality and phosphorus release measurements and a meta-analysis of 230 ponds in the Twin Cities metro area. Other outcomes included a working definition of a constructed stormwater pond and a wetland treating stormwater in the framework of water-body regulations, the development of recommendations for stormwater pond maintenance and wetland management, and an update to the sections on the constructed stormwater ponds section of the 2009 Stormwater Maintenance BMP Guide.

This Technical Summary pertains to Report 2023-25, “Stormwater Pond Maintenance and Wetland Management for Phosphorus Retention,” published June 2023.

Road construction in northeast Minnesota often causes wetland impacts that require compensatory mitigation. Borrow areas excavated for road construction material can be developed into wetland mitigation sites if hydric vegetation, hydric soils and adequate hydrology are provided. Fourteen wetland mitigation sites were constructed north of Virginia, Minnesota along the U.S. Trunk Highway 53 reconstruction project corridor. The sites were established with the goal of mitigating for project impacts to seasonally flooded basin, fresh meadow, shallow marsh, shrub swamp, wooded swamp, and bog wetlands. Monitoring results indicate that the 14 mitigation sites range in their potential to receive wetland mitigation credit. All but one of the sites consistently meet wetland hydrology criteria. The sites contain a variety of plant communities dominated by wet meadow, sedge meadow, and shallow marsh. Floristic Quality Assessment (FQA) condition categories for the sites range from "Poor" to "Exceptional." Invasive plant species, particularly reed canary grass and narrow leaf cattail, are present on a number of sites and should be controlled. Tamarack and black spruce plantings have been successful on some of the drier areas and should be expanded to increase the quality and potential mitigation credit for other sites. These sites have shown the potential for creating mitigation wetlands in abandoned borrow pits in conjunction with highway construction. Adaptive management, particularly water level regulation, early invasive species control, tree planting, and continued long-term annual monitoring can make mitigation sites like these successful options for wetland mitigation credit. Continued site monitoring to determine potential for mitigation credit is recommended.

Human activities including agricultural cultivation, forest harvesting, land development for residential housing, and development for manufacturing and industrial activities can impair the quality of water entering the wetland, thereby detrimentally affecting the natural ecological functions of the wetlands. This can lead to degradation of biota health and biodiversity within the wetland, reduced water quality in the wetland, and increased release of water quality degrading chemicals to receiving waters. Under natural conditions wetlands develop buffer areas that provide some protection from the natural processes occurring on adjacent areas of the landscape. Buffers serve the function of enhancing infiltration of surface runoff generated on adjacent areas, thereby promoting the retention of nutrients in the soil, and retention of sediment suspended in the runoff water, while still allowing runoff water to reach the wetland through subsurface flow routes. To protect wetlands and receiving waters downstream from the wetlands it is important that wetlands in areas disturbed by human activities be provided with sufficient buffer to prevent degradation of wetland biotic integrity as well as degradation of wetland water quality. The question arises, "How much buffer is sufficient?" The objective of this study was to investigate the sufficiency of buffers to protect wetland biotic integrity and water quality, and to evaluate the benefits extended to wildlife by the habit available in wetland buffers. The study was conducted by using a wetland data base available for 64 wetlands in the Twin Cities metro area.

It is becoming increasingly difficult to provide on-site mitigation for wetland impacts due to road construction in northeastern Minnesota counties that retain greater than 80 percent of their pre-settlement wetlands. Abandoned gravel pits are one of the few remaining areas that can serve as wetland mitigation sites. The overall goal of the project is to develop cost-effective methods for creating functional mitigation wetlands on abandoned gravel pit sites to compensate for wetland impacts due to road construction. Two approximately 1-hectare wetland creation demonstration sites were established in adjacent abandoned gravel pits within the U.S. Trunk Highway 53 reconstruction corridor to evaluate techniques for wetland establishment. Wet meadow and shrub swamp wetlands were attempted on one site, and wooded swamp and bog wetlands on the other. Wetland seed mixes provided both positive and negative effects on the developing plant communities on both sites initially but their effect was limited to the first year. Alder thicket and bog donor soil applications had positive effects but not until the third year of the study. Hardwood willow cuttings were effective for establishing a shrub component. Conifer seedlings did not survive unless planted on soil mounds. Fertilizer proved ineffective for promoting wetland plant growth during the study period. The use of straw mulch is questionable on saturated wetland sites such as those in this study.

Phalaris arundinacea invades sedge meadow restorations, forming persistent monotypes that prevent community establishment. Eradicating Phalaris, however, leaves restored ecosystems prone to reinvasion. In order to restore desired plant communities, methods to control Phalaris are needed. To determine if reducing light by sowing cover crops and reducing nitrogen by incorporating soil-sawdust amendments would prevent Phalaris invasions, a study was conducted under conditions similar to a restored wetland in two experimental basins with controlled hydrology. Seeds of a 10-species target community and Phalaris were sown in plots with high diversity, low diversity, or no cover crops in soils with or without sawdust amendments. Nitrogen, light, tissue C:N ratios, firstyear seedling emergence, establishment, and growth, and second-year above ground biomass were measured. Only high diversity cover crops reduced light and sawdust reduced nitrogen for about 9 weeks. Similar trends in firstyear seedling data and second-year biomass data suggested Phalaris control efforts should focus on establishing perennial communities rather than implementing separate resource-limiting strategies. Sowing high diversity cover crops resulted in Phalaris-dominated communities, making cover crops an ineffective Phalaris control strategy. Using sawdust amendments did not reduce Phalaris invasion much beyond what the target community did but resulted in a community similar to those of natural sedge meadows by increasing the abundance of seeded species from the Cyperaceae family and colonization of non-seeded wetland species. The target community apparently reduced Phalaris invasion by reducing both light and nitrogen. Regardless, no treatment fully prevented invasion, making follow-up Phalaris control necessary to ensure community recovery.

The three objectives of this project were as follows: (1.) to survey the arbuscular mycorrhizal fungi from the prairies, wetlands, and Mn/DOT experimental sites in anticipation of using data as indicators of the sites' health, (2.) to produce mycorrhizal inocula by different methods and (3.) to incorporate the inoculum into a Mn/DOT restoration site. This project was the first year of a three year study that seeks to define the variability in the mycorrhizal spores and other structures present in different ecosystems and determine if these fungi can be used as indicator species. All phases of the project were successful. The preliminary results suggest the mycorrhizal fungal spore numbers will be useful health indicators of restoration sites. General and single spore mycorrhizal inocula were generated using primarily native grasses and forbs as plant hosts. The general inoculum was then incorporated into a Mn/DOT experimental site. Future evaluations of this site will help to answer long-term questions regarding the possible benefits of amending disturbed roadway plantings or other prairie restorations with mycorrhizal inoculum.

In this project, researchers studied mycorrhizal and vegetational characteristics at prairie and wetland restoration areas. Study objectives included the following: * quantifying the effect of fungal inoculum on plant communities at a Minnesota Department of Transportation (Mn/DOT) prairie restoration site near Cambridge, Minn. * evaluating the prairie forb germination rates * monitoring revegetation at prairie and wetland restoration sites * characterizing mycorrhizal status of native wetland and prairie areas for comparison to the restored sites * producing fungal inoculum for incorporation into further reclamation areas. Findings indicated that 15 months after planting, fungal inoculation resulted in significantly greater cover by native plant species than seen in control plots. At this site, mycorrhizal inoculation benefited the prairie restoration effort by encouraging earlier, more extensive establishment of the planted species. Ongoing studies at this site will determine the long-term effects of mycorrhizal inoculation on the plant community. The report also presents specific recommendations for future restoration efforts. The studies of mycorrhizae in native prairies and wetlands provide further data for a baseline against which to compare restored areas. In addition, fungal inoculum produced in this project has been incorporated into restoration plots at another Mn/DOT site.